人妻有码中文字幕-国产视频一区二区-99热在线观看-在厨房拨开内裤进入毛片

技術文章您現在的位置:首頁 > 技術文章 > 技術文獻:試劑-藏紅O,固綠FCF引用文獻

技術文獻:試劑-藏紅O,固綠FCF引用文獻

更新時間:2020-12-30   點擊次數:1826次

【文獻標題】Ryanodine receptor 1 mediated dexamethasone-induced chondrodysplasia in fetal rats

【作者】Yi-zhong Wang,Qing-xian Li,Ding-Mei Zhang,et.al

【作者單位】武漢大學中南醫院(Zhongnan Hospital of Wuhan University)

【文獻中引用產品】

試劑-藏紅O

試劑-固綠FCF

【關鍵詞】Chondrodysplasia,Prenatal dexamethasone exposure,Ryanodine receptor 1,Transforming growth factor β,Endoplasmic reticulum stress

【DOI】doi.org/10.1016/j.bbamcr.2020.118791

【影響因子(IF)】4.66

出版期刊】《BBA - Molecular Cell Research》

【產品原文引用】

Chemicals and reagents

Dex was obtained from Shuanghe Pharmaceutical Company (Wuhan, China). RYR1 agonist 4-chloro-3-ehylphenol (4-CEP) was purchased from Macklin (Shanghai, China), and RYR1 inhibitor dantrolene (Dan), ERS agonist tunicamycin (Ti), ERS inhibitor 4-phenylbutyric acid (4-PBA) and Fura-2 AM calcium fluorescent probe were purchased from Meilunbio Biotech (Dalian, China). Fluo-3 AM calcium fluorescent probe was purchased from Beyotime (Shanghai, China).DMEM F12 medium was purchased from Gibco Co., Ltd. (Grand Island,NY, USA). Safranin O and fast green was obtained from Hengyuan Biotech (Shanghai, China). TRIZOL was purchased from Invitrogen (Carlsbad, CA, USA). A reverse transcription and real-time quantitative polymerase chain reaction (RT-qPCR) kit was purchased from Takara Biotechnology Co., Ltd. (Dalian, China). SYBR Green dye was obtained from Applied Biosystems, by Thermo Fisher Scientific (ABI, Foster City,CA, USA). All primers were synthesized by Sangon Biotech (Shanghai,China). Primary antibodies for RYR1 and 78 kDa glucose-regulated protein (GRP78) came from Sigma (St. Louis, MO, USA). The antibody for phosphorylated eukaryotic initiation factor 2A (p-EIF2A) was acquired from CST (Boston, MA, USA), the antibody for ATF-6 was acquired from Santa Cruz (Santa Cruz, CA, USA), the antibody for COL2A1 was acquired from Abbkine (Santa Cruz, CA, USA) and the antibody for ACAN was acquired from Bioss (Beijing, China). TGFβR1 antibody was purchased from ELK Biotechnology (Wuhan, China), and the antibodies for SMAD family member 2 (SMAD2), sex-determining region Y-box 9 protein (SOX9), ADAM metallopeptidase with thrombospondin type 1 motif 5 (ADAMTS5), and matrix metallopeptidase 13 (MMP13) were acquired from abcam (Cambridge, UK). The other reagents for the experiments were of analytical grade.

完整版PDF文獻請咨詢在線客服或者聯系我司業務員!

更多公司福利請關注“恒遠生物”VX公眾號!




主站蜘蛛池模板: 三级特黄60分钟在线观看 | jαpαnesehd熟女熟妇伦| 都市 亚洲 自拍 小说 校园| 国产精品国产三级国产av′| 人妻夜夜添夜夜无码av| 人妻去按摩店被黑人按中出| 国产乱人伦精品一区二区| 青青草原亚洲| 内射夜晚在线观看| 妓女爽爽爽爽爽妓女8888| 女人脱了内裤趴开腿让男生摸 | 国产在线观看免费视频软件| 成年女人永久免费看片| 美女脱了内裤张开腿让男人桶网站| 无码人妻精品一区二区三18禁| 亚洲jlzzjlzz少妇| 天天澡天天添天天摸97影院| 日韩欧美激情兽交| 无码手机线免费观看| 爱情岛论坛首页永久入口| 业余 自由 性别 成熟视频 视频| 国产精品久久无码一区| 国产精品久久二区二区| 精品国产亚洲av麻豆尤物| 国产乱人视频在线播放| 爱情岛论坛亚洲品质自拍hd| 丁香五香天堂网| 成人欧美日韩一区二区三区| 大陆极品少妇内射aaaaaa| 老少交欧美另类| 国产精品后入内射日本在线观看| 久久久久久妓女精品影院| 欲香欲色天天天综合和网| 久久久亚洲精品无码| 亚州少妇无套内射激情视频| 亚洲精品无码久久久久去q| 青草影院内射中出高潮| 中文字幕久久久久人妻| 成人免费无码视频在线网站| 免费人成在线视频无码| 亲子乱aⅴ一区二区三区下载|